Column Chromatography
(โครมาโตกราฟีแบบคอลัมน์)
Definition
Column Chromatography (โครมาโตกราฟีแบบคอลัมน์) Hard Skill
A laboratory technique used to separate and purify individual components from a mixture by passing it through a column packed with a stationary phase, enabling analysis or further use of the separated substances.
Expertise Level
Level 1
Basic
1. Understands the basic principle of column chromatography.
2. Can set up a simple column with stationary phase material.
3. Can load samples and perform simple separations under supervision.
Level 2
Intermediate
1. Can optimize the choice of stationary and mobile phases for better separation.
2. Able to monitor and adjust flow rates during the process.
3. Can identify fractions containing target compounds and evaluate purity.
Level 3
Advanced
1. Designs and optimizes complex column chromatography protocols for challenging separations.
2. Utilizes advanced techniques to increase resolution and recovery.
3. Analyzes results critically to troubleshoot and improve purification processes.
