ELISA
(เอลลิซา)
Definition
ELISA (เอลลิซา) Hard Skill
ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory technique used to detect and quantify specific proteins, antibodies, or antigens in a sample through enzyme-mediated color change reactions.
Expertise Level
Level 1
Basic
1. Understands the basic principles and purpose of ELISA.
2. Can prepare standard reagents and basic solutions needed for the assay.
3. Familiar with the steps involved in performing a standard ELISA test.
Level 2
Intermediate
1. Can accurately perform ELISA assays including sample preparation and dilution.
2. Able to operate ELISA plate readers and interpret absorbance data.
3. Recognizes common errors and can troubleshoot problems during the assay.
Level 3
Advanced
1. Designs and optimizes ELISA protocols for complex or novel assays.
2. Can analyze and validate quantitative ELISA data with statistical methods.
3. Troubleshoots advanced assay issues and adapts protocols to improve sensitivity and specificity.
