Gel Electrophoresis
(การทำเจลอิเล็กโตรโฟรีซิส)
Definition
Gel Electrophoresis (การทำเจลอิเล็กโตรโฟรีซิส) Hard Skill
Gel electrophoresis is a laboratory technique used to separate mixtures of DNA, RNA, or proteins based on their size and charge by applying an electric field through a gel matrix.
Expertise Level
Level 1
Basic
1. Understands the basic principle of gel electrophoresis.
2. Can prepare simple agarose gels.
3. Can load DNA samples into gel wells with guidance.
Level 2
Intermediate
1. Can prepare and run agarose or polyacrylamide gels independently.
2. Understands the impact of voltage, gel concentration, and run time on separation.
3. Can visualize and analyze band patterns using staining techniques.
Level 3
Advanced
1. Can optimize gel electrophoresis conditions for different biomolecules.
2. Able to troubleshoot and resolve complex issues during gel preparation and running.
3. Can interpret electrophoresis results to support experimental conclusions and present findings.
