PCR Primer Design
(การออกแบบไพรเมอร์ PCR)
Definition
PCR Primer Design (การออกแบบไพรเมอร์ PCR) Hard Skill
The skill of creating short DNA sequences (primers) that initiate the polymerase chain reaction (PCR) to amplify specific DNA segments accurately and efficiently.
Expertise Level
Level 1
Basic
1. Understands the basic principles of PCR and primer function.
2. Can identify target DNA sequences for primer design.
3. Uses simple guidelines to select primer sequences manually or with basic software.
Level 2
Intermediate
1. Designs primers considering factors like melting temperature, GC content, and specificity.
2. Uses specialized software tools to optimize primer sequences.
3. Evaluates primer efficiency and potential secondary structures.
Level 3
Advanced
1. Expertly designs primers for multiplex PCR and complex templates.
2. Integrates knowledge of primer-dimer formation, cross-reactivity, and advanced thermodynamics.
3. Validates primer designs experimentally and revises designs based on results.
Ministry of Higher Education
Science, Research and Innovation
Call Center 1313
328 Si Ayutthaya Rd., Thung Phaya Thai, Ratchathewi, Bangkok 10400 Tel. 02-610-5200 Fax. 02-354-5524.
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