Real-Time Polymerase Chain Reaction (qPCR)
(ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเรียลไทม์ (qPCR))
Definition
Real-Time Polymerase Chain Reaction (qPCR) (ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเรียลไทม์ (qPCR)) Tool
Real-Time Polymerase Chain Reaction (qPCR) is a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule, enabling the detection and measurement of nucleic acids in real time during the amplification process.
Expertise Level
Level 1
Basic
1. Understands the basic principles of PCR and qPCR.
2. Can prepare reagents and set up simple qPCR experiments with supervision.
3. Familiar with common qPCR instruments and workflow.
Level 2
Intermediate
1. Independently designs and optimizes qPCR assays for specific targets.
2. Can analyze qPCR data, including melting curves and amplification plots.
3. Troubleshoots common issues in qPCR experiments and maintains equipment.
Level 3
Advanced
1. Develops complex qPCR protocols for multiplex or high-throughput applications.
2. Interprets quantitative data to make critical decisions in research or diagnostics.
3. Leads troubleshooting, validation, and training on advanced qPCR techniques.
