Reverse Transcription Polymerase Chain Reaction (RT-PCR)
(ปฏิกิริยาโพลีเมอเรสแบบลูปย้อนกลับ (RT-PCR))
Definition
Reverse Transcription Polymerase Chain Reaction (RT-PCR) (ปฏิกิริยาโพลีเมอเรสแบบลูปย้อนกลับ (RT-PCR)) Hard Skill
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a laboratory technique used to convert RNA into complementary DNA (cDNA) and then amplify specific DNA sequences to analyze gene expression or detect RNA viruses.
Expertise Level
Level 1
Basic
1. Understands the basic principle of RT-PCR and its purpose.
2. Can prepare simple RT-PCR reaction mixtures with supervision.
3. Familiar with common reagents and basic laboratory equipment used in RT-PCR.
Level 2
Intermediate
1. Can independently perform RNA extraction and reverse transcription steps.
2. Able to set up and run RT-PCR experiments following protocols accurately.
3. Understand how to interpret RT-PCR results and identify common errors.
Level 3
Advanced
1. Designs and optimizes RT-PCR protocols for various RNA targets and applications.
2. Troubleshoots complex problems and ensures quality control in RT-PCR experiments.
3. Analyzes quantitative RT-PCR data and applies advanced techniques such as real-time PCR.
