SDS-PAGE
(SDS-PAGE (โซเดียมโดเดซิลซัลเฟต-โพลีอะคริลาไมด์เจลอิเล็กโทรโฟรีซิส))
Definition
SDS-PAGE (SDS-PAGE (โซเดียมโดเดซิลซัลเฟต-โพลีอะคริลาไมด์เจลอิเล็กโทรโฟรีซิส)) Hard Skill
SDS-PAGE is a laboratory technique used to separate proteins based on their molecular weight by applying an electric field to a polyacrylamide gel containing sodium dodecyl sulfate (SDS), which denatures proteins and imparts a uniform negative charge.
Expertise Level
Level 1
Basic
1. Understands the basic principle of SDS-PAGE and its purpose.
2. Can prepare simple SDS-PAGE gels and samples.
3. Follows standard protocols for running SDS-PAGE.
Level 2
Intermediate
1. Can optimize gel concentration and running conditions for different protein sizes.
2. Able to troubleshoot common issues such as smearing or poor resolution.
3. Prepares samples with reducing and non-reducing conditions.
Level 3
Advanced
1. Designs complex SDS-PAGE experiments with gradient gels or specialized buffers.
2. Interprets protein patterns for molecular weight, purity, and post-translational modifications.
3. Integrates SDS-PAGE results with other biochemical or analytical techniques.
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