Western Blotting
(การทำเวสเทิร์นบลอต)
Definition
Western Blotting (การทำเวสเทิร์นบลอต) Hard Skill
Western Blotting is a laboratory technique used to detect specific proteins in a sample by separating them using gel electrophoresis and transferring them onto a membrane for antibody-based detection.
Expertise Level
Level 1
Basic
1. Understands the basic principles and purpose of Western Blotting.
2. Can prepare samples and reagents following standard protocols.
3. Is familiar with gel electrophoresis setup and membrane transfer basics.
Level 2
Intermediate
1. Can independently run gel electrophoresis and transfer proteins to membranes.
2. Able to perform antibody incubations and washing steps accurately.
3. Knows how to optimize conditions for signal detection and reduce background noise.
Level 3
Advanced
1. Can troubleshoot and optimize all steps including sample preparation, transfer efficiency, and antibody specificity.
2. Able to design experiments using Western Blotting for quantitative and qualitative protein analysis.
3. Experienced in interpreting complex results and integrating data into broader research.
